Cell-based assays play a crucial role in the process of drug discovery, toxicity testing and have the potential to contribute to new insights into cellular behavior in various biomedical research areas. However, most of the common available assays have their limitations resulting in low gain of knowledge and erroneous data interpretations and conclusions. Cell Adhesion Noise (CAN)-Spectroscopy overcomes these limitations and exhausts the full potential of cell-based assays by combining the advantages of cell microscopy and impedance spectroscopy.
Briefly, the key features and benefits of CAN-Spectroscopy are:
> Label-free - No interferences with any label, no photo bleaching effects, no artifacts due to a label
> Non-invasive - Cells are analyzed in their native environment
> Multi- time points acquisition - Continuous measurement up to days or weeks is feasible, which enables the performance of kinetics and contribute to a gain of knowledge about the dynamic of the cellular event
> Multi-parametric read-out - Several parameters have been captured per acquisition
> Highly sensitive with a resolution of 6 µm per pixel - Analysis of subcellular structures is feasible
> Correlation of curve and corresponding image - Gives data a higher reliability and improves data interpretation
> Automated data acquisition and analysis - Excluding operator biases
> Without changing any magnification, the subsequent analysis will provide data on cell population, sub-population, single cell or subcellular level
CAN-Spectroscopy facilities an easy-to-perform quantitative real-time analysis on single cell level of various cellular events, wherefore otherwise sophisticated, expensive and labor-intensive technologies have been needed. By performing CAN-Spectroscopy, the captured electrical signals provide simultaneously data about cellular features that are used for an in-depth characterization of:
> Cell adhesion and spreading
> Cell viability & cytotoxicity
> Cell type specific electrical fingerprint
> G protein coupled receptor (GPCR) activation and transduction
In the following, a brief description of the above mentioned cellular events is given with a focus on the applications of CAN-Spectroscopy.
Cell adhesion and cell spreading
Cell adhesion is a highly dynamic process and of major interest in questions regarding tissue development, inflammation, immunity, cancer and many others. The process of adhesion can be divided into three steps: Initial attachment, flatting and spreading. Furthermore, adhesion potential continually increased over time.
ñ Quantification of cell attachment kinetics
ñ Quantification of cell detachment kinetics
ñ Identification of morphological and cytoskeleton changes
ñ Determining of micromotion